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<div><h1>人抗體ELISA試劑盒的原理及應用介紹</h1></div> <div class="pro_details_tongji"> 　更新時間：2023-11-09　點擊量：<span id="Click">41</span> </div> <div class="n_con" id="c_detail_wrap"> <div>　　<strong>人抗體ELISA試劑盒</strong>是一種常用的免疫學實驗方法，用于檢測體內是否存在特定人類抗體的定量分析。其原理基于酶聯免疫吸附法，主要包括固相抗原涂布、樣品孵育、抗體結合和檢測信號等步驟。<br /> </div><div>　　<a href="http://www.lifengnongzhuang.com/Products-37034485.html" target="_blank"><strong>人抗體ELISA試劑盒</strong></a>的基本原理如下：<br /> </div><div>　　首先，將待檢測抗原或抗體固定在微孔板上。這些固相抗原可以是純化的蛋白質、多肽、糖類等，或者是細胞濾液、組織提取物等復雜混合物。通過固定，使抗原或抗體與微孔板表面非特異性地結合。<br /> </div><div>　　隨后，加入待測樣品到微孔中，并讓其充分接觸固相抗原，進行孵育。如果樣品中存在與固相抗原或抗體特異性結合的抗體，它們將與固相抗原結合形成抗原-抗體復合物。<br /> </div><div>　　然后，洗滌孔內未結合的物質，以去除非特異性結合的成分。這個步驟能有效減少假陽性結果的產生，提高試劑盒的特異性。<br /> </div><p align="center"><img src="https://img43.chem17.com/9/20231109/638351336040074101629.png" alt="人抗體ELISA試劑盒" width="372" height="302" align="middle" /></p><p> </p><div>　　接下來，加入與被檢測抗體特異性結合的檢測抗體，并使其與復合物發生特異性結合。這樣，形成了夾心型結構：固相抗原-待測抗體-檢測抗體。再次進行洗滌，以去除未結合的檢測抗體。<br /> </div><div>　　最后，加入一種與檢測抗體結合產生顯色反應的底物，一般是染色底物，常見的有TMB或ABTS。底物在酶的作用下發生化學反應，產生可測量的顏色。<br /> </div><div>　　通過測量反應物顏色的強度，可以定量分析待測樣品中存在的特定人類抗體的濃度。通常使用光密度計或酶標儀對反應顏色進行讀取，并將結果與已知濃度的標準曲線相比較，計算出待測抗體的濃度。<br /> </div><div>　　<strong>人抗體ELISA試劑盒</strong>廣泛應用于許多領域，包括臨床診斷、生物醫藥研究和生物工程等。具體應用包括：<br /> </div><div>　　1、感染性疾病的診斷：ELISA可以檢測感染性疾病引起的抗體產生，用于早期感染篩查和疾病診斷。<br /> </div><div>　　2、自身免疫疾病的診斷：ELISA可檢測自身免疫疾病相關抗體的存在，幫助醫生進行疾病診斷和監測治療效果。<br /> </div> </div> </div> <div class="cl"></div> <div class="ye"> 上一條：沒有了<br />下一條：<a href="/Article-3497614.html">人因子ELISA試劑盒在臨床診斷中的作用和意義</a> </div> </div> </div> </div> <div class="clearfix"></div> <div id="clear"></div>  <footer class="footer"> <div class="wrapper w1200"> <div class="ft-inner"> <div class="leftCon"> <div class="ft-info"> <div class="ft-logo"><img src="/Skins/529073/images/footlogo.png" alt="上?？婆嗳鹕锟萍加邢薰?></div> <div class="ft-tel"> <span>郵箱</span> <p>2582166088@qq.com</p> </div> <div class="ft-address"> <span>地址</span> <p>上海嘉定工業區葉城路912號J</p> </div> </div> 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